Department of Chemistry, University of Alberta
Department of Chemistry, University of Alberta


Sebastianelli L, Kaur H, Chen Z, Krishnamurthy R, Mansy SS (2024) A magnesium binding site and the anomeric effect regulate the abiotic redox chemistry of nicotinamide nucleotides. Chem Eur J. 30, e202400411. DOI: 10.1002/chem.202400411 [link] [data]

Rossetto D, Sebastianelli L, Oberegger S, Todorovic S, Haas H, Mansy SS (2024) Peptide mimics of the cysteine-rich regions of HapX and SreA bind a [2Fe-2S] cluster in vitro. Adv Biol 8, 2300545, DOI:10.1002/adbi.202300545 [link] [data]

Toparlak ÖD, Sebastianelli L, Egas Ortuno V, Karki M, Szostak JW, Krishnamurthy R, Mansy SS (2023) Cyclophospholipids enable a protocellular life cycle. ACS Nano 17, 23772–23783. DOI: 10.1021/acsnano.3c07706 [link] [data]

Liu Y, Liu S, Tomar A, Yen FS, Unlu G, Ropek N, Weber RA, Wang Y, Khan A, Gad M, Peng J, Terzi E, Alwaseem H, Pagano AE, Heissel S, Molina H, Allwein B, Kenny TC, Possemato RL, Zhao L, Hite RK, Vinogradova EV, Mansy SS, Birsoy K (2023) Autoregulatory control of mitochondrial glutathione homeostasis. Science 382, 820-828. DOI: 10.1126/science.adf4154 [link]

Mugoni V, Ciani Y, Quaini O, Tomasini S, Notarangelo M, Vannuccini F, Marinelli A, Leonardi E, Pontalti S, Martinelli A, Rossetto D, Pesce I, Mansy SS, Barbareschi M, Ferro A, Caffo O, Attard G, Di Vizio D, D’Agostino VG, Nardella C, Demichelis F (2023) Integrating extracellular vesicle and circulating cell-free DNA analysis on a single plasma aliquot from breast cancer patients improves the detection of HER2 positivity. J Ex Bio 2, e108. DOI: 10.1002/jex2.108 [link]

Nader S, Baccouche A, Connolly F, Abou-Ghanem M, Styler SA, Lewis J, Pink D, Mansy SS (2023) Model atmospheric aerosols convert to cell-sized vesicles upon entry into lipid coated aqueous solution. ACS Earth Space Chem 7, 252–259. DOI: 10.1021/acsearthspacechem.2c00328 [link] [data]

Valer L, Rossetto D, Parkkila T, Sebastianelli L, Guella G, Hendricks AL, Cowan JA, Sang L, Mansy SS (2022) Histidine ligated iron-sulfur peptides. ChemBioChem 23, e202200202. DOI: 10.1002/cbic.202200202 [link] [data]

Rossetto D, Valer L, Yeh Martín N, Guella G, Hongo Y, Mansy SS (2022) Prebiotic environments with Mg2+ and thiophilic metal ions increase the thermal stability of cysteine and non-cysteine peptides. ACS Earth Space Chem 6, 1221-1226. DOI: 10.1021/acsearthspacechem.2c00042 [link]

Nader S, Sebastianelli L, Mansy SS (2022) Protometabolism as out-of-equilibrium chemistry. Philos Trans R Soc A. 380, 20200423. DOI: 10.1098/rsta.2020.0423 [link]

Peng H, Rossetto D, Mansy SS, Jordan MC, Roos KP, Chen IA (2022) Treatment of wound infections in a mouse model using Zn2+-releasing phage bound to gold nanorods. ACS Nano 16, 4756–4774. DOI: 10.1021/acsnano.2c00048 [link]

Rossetto D, Mansy SS (2022) Metals are integral to life as we know it. Front Cell Dev Biol. 10, 864830. DOI: 10.3389/fcell.2022.864830 [link]

Valer L, Rossetto D, Scintilla S, Tomar A, Nader S, Hu YJ, Betinol IO, Mansy SS (2022) Methods to identify and characterize iron-sulfur oligopeptides in water. Can J Chem. DOI: 10.1139/cjc-2021-0237 [link]

Sebastianelli L, Mansy SS (2022) Origins of life: Encapsulating Darwinian evolution. Curr Biol 32, R44-R46. DOI: 10.1016/j.cub.2021.11.026 [link]

Ha M, Nader S, Pawsey S, Struppe J, Monette M, Mansy SS, Boekhoven J, Michaelis VK (2021) Racing Towards Fast and Effective 17O Isotopic Labeling and NMR Spectroscopy of N-formyl-MLF-OH and Associated Building Blocks. J Phys Chem B 125, 11916–11926. DOI: 10.1021/acs.jpcb.1c07397 [link]

Betinol IO, Nader S, Mansy SS (2021) Spectral decomposition of iron-sulfur clusters. Anal Biochem 629, 114269. DOI: 10.1016/j.ab.2021.114269 [link] [download Fit-FeS]

Basak S, Nader S, Mansy SS (2021) Protometabolic Reduction of NAD+ with α-Keto Acids. JACS Au 1, 371−374. DOI: 10.1021/jacsau.0c00124 [link] [NMR spectra]

Toparlak ÖD, Wang A, Mansy SS (2021) Population-level Membrane Diversity Triggers Growth and Division of Protocells. JACS Au 1, 560-568. DOI: 10.1021/jacsau.0c00079 [link]

Toparlak ÖD, Zasso J, Bridi S, Dalla Serra M, Macchi P, Conti L, Baudet M-L, Mansy SS (2020) Artificial cells drive neural differentiation. Sci Adv 6, eabb4920. DOI: 10.1126/sciadv.abb4920 [link] [UofA press] [Edmonton Journal] [Radio Canada]

Groaz A, Galvan S, Valer L, Rossetto D, Benedetti F, Guella G, Toparlak ÖD, Mansy SS (2020) Cell-free synthesis of dopamine and serotonin in two steps with purified enzymes. Adv Biosyst 4, 2000118. DOI: 10.1002/adbi.202000118 [link]

Toparlak ÖD, Karki M, Egas Ortuno V, Krishnamurthy R, Mansy SS (2020) Cyclophospholipids Increase Protocellular Stability to Metal Ions. Small 16, 1903381. DOI: 10.1002/smll.201903381 [link] [press]

Yeh Martín N, Valer L, Mansy SS (2019) Toward long-lasting artificial cells that better mimic natural living cells. Emerging Top Life Sci 3, 597-607. DOI: 10.1042/ETLS20190026 [link]

Toparlak ÖD, Mansy SS (2019) Progress in synthesizing protocells. Exp Biol Med 244, 304-313. DOI: 10.1177/1535370218816657 [link]

Bonfio C, Godino E, Corsini M, Fabrizi De Biani F, Guella G, Mansy SS (2018) Prebiotic iron-sulfur peptide catalysts generate a pH gradient across model membranes of late protocells. Nat Catal 1, 616-623. DOI: 10.1038/s41929-018-0116-3 [link]

Schwarz-Schilling M, Dupin A, Chizzolini F, Krishnan S, Mansy SS, Simmel FC (2018) Optimized assembly of a multi-functional RNA-protein nanostructure in a cell-free gene expression system. Nano Lett 18, 2650-2657. DOI: 10.1021/acs.nanolett.8b00526 [link]

Sen S, Bonfio C, Mansy SS, Cowan JA (2018) Investigation of glutathione-derived electrostatic and hydrogen-bonding interactions and their role in defining Grx5 [2Fe–2S] cluster optical spectra and transfer chemistry. J Biol Inorg Chem 23, 241-252. DOI: 10.1007/s00775-017-1525-5 [link]

Rhoads MK, Hauk P, Terrell J, Tsao C-Y, Oh H, Raghavan SR, Mansy SS, Payne GF, Bentley WE (2018) Incorporating LsrK AI-2 Quorum Quenching Capability in a Functionalized Biopolymer Capsule. Biotechnol Bioeng 115, 278-289. DOI: 10.1002/bit.26397 [link]

Tang T-YD, Cecchi D, Fracasso G, Accardi D, Coutable A, Mansy SS, Perriman AW, Anderson JLR, Mann S (2018) Gene-mediated chemical communication in synthetic protocell communities. ACS Synth Biol 7, 339–346. DOI: 10.1021/acssynbio.7b00306 [link]

Bonfio C, Valer L, Scintilla S, Shah S, Evans DJ, Jin L, Szostak JW, Sasselov DD, Sutherland JD, Mansy SS (2017) UV light-driven prebiotic synthesis of iron-sulfur clusters. Nat Chem 9, 1229–1234. DOI: 10.1038/nchem.2817 [link]

Belmonte L, Mansy SS (2017) Patterns of Ligands Coordinated to Metallocofactors Extracted from the Protein Data Bank. J Chem Inf Model 57, 3162–3171. DOI: 10.1021/acs.jcim.7b00468 [link]

Lentini R, Yeh Martin N, Forlin M, Belmonte L, Fontana J, Martini L, Cornella M, Tamburini S, Bentley WE, Jousson O, Mansy SS (2017) Two-way chemical communication between artificial and natural cells. ACS Cent Sci 3, 117-123. DOI: 10.1021/acscentsci.6b00330 [link]

Mansy SS (2017) Protocells: Non-living predators. Nat Chem 9, 107-108. DOI: 10.1038/nchem.2725 [link]

Bonfio C, Mansy SS (2017) The chemical roots of iron-sulfur dependent metabolism. Biochemistry 56, 5225–5226. DOI: 10.1021/acs.biochem.7b00842 [link]

Chizzolini F, Forlin M, Yeh Martín N, Berloffa G, Cecchi D, Mansy SS (2017) Cell-free translation is more variable than transcription. ACS Synth Biol 6, 638–647. DOI: 10.1021/acssynbio.6b00250 [link]

Scintilla S, Bonfio C, Belmonte L, Forlin M, Rossetto D, Li J, Cowan JA, Galliani A, Arnesano F, Assfalg M, Mansy SS (2016) Duplications of an Iron-Sulphur Tripeptide Leads to the Formation of a Protoferredoxin. Chem Commun 52, 13456-13459. DOI: 10.1039/C6CC07912A [link]

Lentini R, Yeh Martin N, Mansy SS (2016) Communicating artificial cells. Curr Opin Chem Biol 34, 53-61. DOI: 10.1016/j.cbpa.2016.06.013 [link]

Belmonte L, Rossetto D, Forlin M, Scintilla S, Bonfio C, Mansy SS (2016) Cysteine containing dipeptides show a metal specificity that matches the composition of seawater. Phys Chem Chem Phys 18, 20104-20108. DOI: 10.1039/C6CP00608F [link]

Belmonte L, Mansy SS (2016) Metal catalysts and the origin of life. Elements 12, 413-418. DOI: 10.2113/gselements.12.6.413 [link]

Beal J, Haddock-Angelli T, Gershater M, de Mora K, Lizarazo M, Hollenhorst J, Rettberg R, iGEM Interlab Study Contributors (2016) Reproducibility of Fluorescent Expression from Engineered Biological Constructs in E. coli. PLoS ONE 11, e0150182. doi:10.1371/journal.pone.0150182 [link]

Martini L, Ellington AD, Mansy SS (2016) An in vitro selection for small molecule induced switching RNA molecules. Methods 106, 51-57. DOI: 10.1016/j.ymeth.2016.02.010 [link]

Martini L, Meyer AJ, Ellefson JW, Milligan JN, Forlin M, Ellington AD, Mansy SS (2015) In vitro selection for small molecule triggered strand displacement and riboswitch activity. ACS Synth Biol 4, 1144–1150. DOI: 10.1021/acssynbio.5b00054 [link]

Lentini R, Santero SP, Chizzolini F, Cecchi D, Fontana J, Marchioretto M, Del Bianco C, Terrell JL, Spencer AC, Martini L, Forlin M, Assfalg M, Dalla Serra M, Bentley WE, Mansy SS (2014). Integrating artificial with natural cells to translate chemical messages that direct E. coli behaviour. Nat Commun 5, 4012. DOI: 10.1038/ncomms5012 [link]

Digiacomo F, Girelli G, Bruno A, Marchioretti C, Pedrotti M, Perli T, Tonon E, Valentini V, Avi D, Ferrentino G, Dorigato A, Jousson O, Mansy SS, Del Bianco C (2014) Ethylene-producing bacteria that ripen fruit. ACS Synth Biol 3, 935-938. DOI: 10.1021/sb5000077 [link]

Del Bianco C, Torino D, Mansy SS (2014) Vesicle preparation and stability: an undergraduate laboratory. J Chem Educ 91, 1228–1231. DOI: 10.1021/ed400105q [link]

Torre P, Keating CD, Mansy SS (2014) Multiphase water-in-oil emulsion droplets for cell-free transcription-translation. Langmuir 30, 5695-5699. DOI: 10.1021/la404146g [link]

Chizzolini F, Forlin M, Cecchi D, Mansy SS (2014) Gene position more strongly influences cell-free protein expression from operons than T7 transcriptional promoter strength. ACS Synth Biol 3, 363-371. DOI: 10.1021/sb4000977 [link]

Martini L, Mansy SS (2014) Measuring riboswitch activity in vitro and in artificial cells with purified transcription-translation machinery. Methods Mol Biol 1111, 153-164. DOI: 10.1007/978-1-62703-755-6_11 [link]

Spencer AC, Torre P, Mansy SS (2013) The encapsulation of cell-free transcription and translation machinery in vesicles for the construction of cellular mimics. J Vis Exp 80, e51304, doi:10.3791/51304 [link]

Lentini R, Forlin M, Martini L, Del Bianco C, Spencer AC, Torino D, Mansy SS (2013) Fluorescent proteins and in vitro genetic organization for cell-free synthetic biology. ACS Synth Biol 2, 482–489. DOI: 10.1021/sb400003y [link]

Torino D, Martini L, Mansy SS (2013) Piecing together cell-like systems. Curr Org Chem 17, 1751-1757 [link]

Forlin M, Lentini R, Mansy SS (2012) Cellular Imitations. Curr Opin Chem Biol 16, 586-592 [link]

Del Bianco C, Mansy SS (2012) Nonreplicating protocells. Acc Chem Res 45, 2125-2130 [link]

Martini L, Mansy SS (2011) Cell-like systems with riboswitch controlled gene expression. Chem Commun 47, 10734-10736. DOI: 10.1039/C1CC13930D [link]

Wu S-P, Bellei M, Mansy SS, Battistuzzi G, Sola M, Cowan JA (2011) Redox chemistry of the Schizosaccharomyces pombe ferredoxin electron-transfer domain and influence of Cys to Ser substitutions. J Inorg Biochem 105, 806-811. DOI: 10.1016/j.jinorgbio.2011.03.004 [link]

Torino D, Del Bianco C, Ross LA, Ong JL, Mansy SS (2011) Intravesicle isothermal DNA replication. BMC Res Notes 4, 128

Mansy SS (2010) Experimental model protocells support a heterotrophic origin of life. Orig Life Evol Biosph 40, 394-397.

Bellei M, Battistuzzi G, Wu S-P, Mansy SS, Cowan JA, Sola M (2010) Control of reduction thermodynamics in [2Fe-2S] ferredoxins. Entropy-enthalpy compensation and the influence of surface mutations. J Inorg Biochem 104, 691-696. DOI: 10.1016/j.bpj.2009.09.025 [link]

Bruckner RJ, Mansy SS, Ricardo A, Mahadevan L, Szostak JW (2009) Flip-flop induced relaxation of the bending energy in synthetic and biological membranes. Biophys J 97, 3113-3122 [link]

Mansy SS, Szostak JW (2009) Reconstructing the Emergence of Cellular Life through the Synthesis of Model Protocells. Cold Spring Harb Symp Quant Biol 74, 47-54.DOI: 10.1101/sqb.2009.74.014 [link]

Mansy SS (2009) Model Protocells from Single-Chain Lipids. Int J Mol Sci 10, 835-843 [link]

Mansy SS, Szostak JW (2008) Thermostability of Model Protocell Membranes. Proc Natl Acad Sci USA 105, 13351-13355. DOI: 10.1073/pnas.0805086105 [link]

Mansy SS, Schrum JP, Krishnamurthy M, Tobé S, Treco D, Szostak JW (2008) Template-Directed Synthesis of a Genetic Polymer inside of a Model Protocell. Nature 454, 122-125. DOI:10.1038/nature07018 [link]

Mansy SS, Zhang J, Kümmerle R, Nilsson M, Chou JJ, Szostak JW, Chaput JC (2007) Structure and Evolutionary Analysis of a Non-Biological ATP-Binding Protein. J Mol Biol 371, 501-513. DOIL 10.1016/j.jmb.2007.05.062 [link]

Hanczyc M, Mansy SS, Szostak JW (2007) Mineral surface directed membrane assembly. Orig Life Evol Biosph 37, 67-82. DOI: 10.1007/s11084-006-9018-5 [link]

Wu S-P, Mansy SS, Cowan JA (2005) Iron-Sulfur Cluster Biosynthesis. Molecular chaperone DnaK promotes IscU-bound [2Fe-2S] cluster stability and inhibits cluster transfer activity. Biochemistry 44, 4284-4293. DOI: 10.1021/bi0483007 [link]

Mansy SS, Cowan JA (2004) Iron-Sulfur Cluster Biosynthesis. Toward an Understanding of Cellular Machinery and Molecular Mechanism. Acc Chem Res 37, 719-725. DOI: 10.1021/ar0301781 [link]

Mansy SS, Wu S-P, Cowan JA (2004) Iron-Sulfur Cluster Biosynthesis. Biochemical Characterization of the Conformational Dynamics of Thermotoga maritima IscU and the Relevance for Cellular Cluster Assembly. J Biol Chem 279, 10469-10475. DOI: 10.1074/jbc.M312051200 [link]

Venturoli G, Mamedov M, Mansy SS, Musiani F, Strocchi M, Francia F, Semenov AY, Cowan JA, Ciurli S (2004) Electron transfer from HiPIP to the Photo-Oxidized Tetraheme Cytochrome Subunit of Allochromatium vinosum Reaction Center. New insights from Site-Directed Mutagenesis and Computational Studies. Biochemistry 43, 437-445. DOI: 10.1021/bi035384v [link]

Bertini I, Cowan JA, Del Bianco C, Luchinat C, Mansy SS (2003) Thermotoga maritima IscU. Structural Characterization and Dynamics of a New Class of Metallochaperone. J Mol Biol 331, 907-924. DOI: 10.1016/S0022-2836(03)00768-X [link]

Mansy SS, Wu G, Surerus KK, Cowan JA (2002) Iron-Sulfur Cluster Biosynthesis: Thermatoga maritima IscU is a Structured Iron-Sulfur Cluster Assembly Protein. J Biol Chem 277, 21397-21404. DOI: 10.1074/jbc.M201439200 [link]

Wu G, Mansy SS, Hemann C, Hille R, Surerus KK, Cowan JA (2002) Iron-Sulfur Cluster Biosynthesis: Characterization of Schizosaccaromyces pombe Isa1. J Biol Inorg Chem 7, 526-532. DOI: 10.1007/s00775-001-0330-2 [link]

Wu G, Mansy SS, Wu S-P, Surerus KK, Foster MW, Cowan JA (2002) Characterization of an Iron-sulfur Cluster Assembly Protein (ISU1) from Schizosaccaromyces pombe. Biochemistry 41, 5024-5032. DOI: 10.1021/bi016073s [link]

Mansy SS, Xiong Y, Hemann C, Hille R, Sundaralingam M, Cowan JA (2002) Crystal Structure and Stability Studies of C77S HiPIP: A Serine Ligated [4Fe-4S] Cluster. Biochemistry 41, 1195-1201. DOI: 10.1021/bi011811y [link]

Foster MW, Mansy SS, Hwang J, Penner-Hahn JE, Surerus KK, Cowan JA (2000) A Mutant Human IscU Protein Contains a Stable [2Fe-2S]2+ Center of Possible Functional Significance. J Am Chem Soc 122, 6805-6806. DOI: 10.1021/ja000800+ [link]

Gong W, Hao B, Mansy SS, Gonzalez G, Gilles-Gonzalez MA, Chan MK (1998) Structure of a Biological Oxygen Sensor: A New Mechanism for Heme-Driven Signal Transduction. Proc Natl Acad Sci USA 95, 15177-15182. DOI: 10.1073/pnas.95.26.15177 [link]

Mansy SS, Olson JS, Gonzalez G, Gilles-Gonzalez MA (1998) Imidazole is a Sensitive Probe of Steric Hindrance in the Distal Pockets of Oxygen-Binding Heme Proteins. Biochemistry 37, 12452-12457. DOI: 10.1021/bi980516j [link]